Energieopslag-enzym

Does ENOblock inhibit glycolytic enzyme enolase?

ENOblock does not inhibit the activity of the glycolytic enzyme enolase.

How do E1 enzymes activate UBLs?

E1 enzymes activate UBLs through carboxy-terminal adenylation and thiol transfer. They then coordinate the use of UBLs in specific downstream pathways by charging cognate E2 enzymes, which interact with the downstream ubiquitylation machinery to coordinate the modification of the target.

What is the E1 enzyme for SUMO?

The E1 enzyme for SUMO is SUMO-activating enzyme 1 (SAE1)–UBA2.

How does Arg 374 affect Lys 345 in enolase?

Arg 374 causes Lys 345 in the active site to become deprotonated, which primes Lys 345 for its role in the mechanism. In recent medical experiments, enolase concentrations have been sampled in an attempt to diagnose certain conditions and their severity.

Why are E1 enzymes unique in the Ubl conjugating pathway?

E1 enzymes are unique in the UBL conjugating pathway in that they are the only components that use ATP. This property is being exploited to develop selective inhibitors. The potential for the use of such inhibitors in therapy is high, given the links that are seen between the components of UBL cascades and human disease.

How does enolase work?

In human α-enolase, the substrate is rotated into position upon binding to the enzyme due to interactions with the two catalytic magnesium ions, Gln 167, and Lys 396. Movements of loops Ser 36 to His 43, Ser 158 to Gly 162, and Asp 255 to Asn 256 allow Ser 39 to coordinate with Mg 2+ and close off the active site.